Price range: $268.00 through $328.00
Background:The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Mar 2013],
| Size | 50μL/100μL |
| Target | Arp3 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 47kD |
| MW(Observed) | 47kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1316R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | ACTR3 |
| Protein Name | Actin-related protein 3 |
| Other Name | ACTR3; / ARP3; / Actin-related protein 3; / Actin-like protein 3 |
| Species | Human |
| Gene ID | 10096 |
| Uniprot | P61158, |
| Species.1 | Mouse |
| Gene ID.1 | 74117 |
| Uniprot.1 | Q99JY9, |
| Species.2 | Rat |
| Gene ID.2 | 81732 |
| Uniprot.2 | Q4V7C7 |
| Cellular Localization | Cytoplasm, cytoskeleton . Cell projection . Nucleus . In pre-apoptotic cells, colocalizes with MEFV in large specks (pyroptosomes) (PubMed:19109554). |
| Tissue Expression | Brain,Cajal-Retzius cell,T-cell, |
| Signaling_pathway | Cellular Processes >> Cellular community - eukaryotes >> Tight junction;Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Immune system >> Fc gamma R-mediated phagocytosis |
| Research Areas | >>Endocytosis; / >>Tight junction; / >>Fc gamma R-mediated phagocytosis; / >>Regulation of actin cytoskeleton; / >>Bacterial invasion of epithelial cells; / >>Pathogenic Escherichia coli infection; / >>Shigellosis; / >>Salmonella infection; / >>Yersinia infection |
| Function | Function:Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament.,similarity:Belongs to the actin family.,similarity:Belongs to the actin family. ARP3 subfamily.,subunit:Component of the Arp2/3 complex composed of ARP2, ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC. Interacts with WHDC1., |
