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Home/Reagents/Antibody/Primary antibody/Polyclonal Antibody/Rabbit pAb/CLIC1 Polyclonal Antibody
SKU: WP5886 Categories: ,

CLIC1 Polyclonal Antibody

Ucallm_WP5886-50

Price range: $268.00 through $328.00

SKU: WP5886-100
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Product details

Background:

chloride intracellular channel 1(CLIC1) Homo sapiens Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 1 is a member of the p64 family; the protein localizes principally to the cell nucleus and exhibits both nuclear and plasma membrane chloride ion channel activity. [provided by RefSeq, Jul 2008],

Specifications

Size50μL/100μL
TargetCLIC1
ReactivityHuman, Mouse, Rat
ApplicationsIHC, IF
MW(Calculated)
MW(Observed)30kD
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Recommended Dilution RatioIHC 1:100-200; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenSynthetic Peptide of CLIC1 AA range: 166-216
SpecificityCLIC1 protein(A216) detects endogenous levels of CLIC1
Gene NameCLIC1
Protein NameChloride intracellular channel protein 1 (Chloride channel ABP) (Nuclear chloride ion channel 27) (NCC27) (Regulatory nuclear chloride ion channel protein) (hRNCC)
Other NameChloride intracellular channel protein 1; / Chloride channel ABP; / Nuclear chloride ion channel 27; / NCC27; / Regulatory nuclear chloride ion channel protein; / hRNCC
SpeciesHuman
Gene ID1192
UniprotO00299,
Species.1Mouse
Gene ID.1
Uniprot.1Q9Z1Q5,
Species.2Rat
Gene ID.2
Uniprot.2Q6MG61
Cellular LocalizationNucleus . Nucleus membrane; Single-pass membrane protein . Cytoplasm . Cell membrane; Single-pass membrane protein . Mostly in the nucleus including in the nuclear membrane (PubMed:9139710, PubMed:12681486). Small amount in the cytoplasm and the plasma membrane (PubMed:9139710). Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain (PubMed:11940526, PubMed:11551966, PubMed:14613939). .
Tissue ExpressionExpression is prominent in heart, placenta, liver, kidney and pancreas.
Signaling_pathway
Research Areas
FunctionDomain:Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.,Function:Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions.,miscellaneous:The protein seems to have very low affinity for glutathion, even though glutathion binding was observed in protein crystals.,PTM:Hydrogen peroxide treatment causes a conformation change, leading to dimerization and formation of an intramolecular disulfide bond between Cys-24 and Cys-59.,similarity:Belongs to the chloride channel CLIC family.,similarity:Contains 1 GST C-terminal domain.,subcellular location:Mostly in the nucleus including in the nuclear membrane. Small amount in the cytoplasm and the plasma membrane. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain.,subunit:Monomer. Homodimer (in vitro). Interacts with TRAPPC2. Dimerization requires a conformation change that leads to the exposure of a large hydrophobic surface. In vivo, this may lead to membrane insertion.,
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