Price range: $268.00 through $328.00
Background:
A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | CSPG4 |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC, IF |
| MW(Calculated) | |
| MW(Observed) | 255kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:50-300; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol,0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from human protein . at AA range: 1910-1990 |
| Specificity | CSPG4 Polyclonal Antibody detects endogenous levels of protein. |
| Gene Name | CSPG4 MCSP |
| Protein Name | Chondroitin sulfate proteoglycan 4 (Chondroitin sulfate proteoglycan NG2) (Melanoma chondroitin sulfate proteoglycan) (Melanoma-associated chondroitin sulfate proteoglycan) |
| Other Name | |
| Species | Human |
| Gene ID | 1464 |
| Uniprot | Q6UVK1, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q8VHY0, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | Q00657 |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein; Extracellular side . Apical cell membrane; Single-pass type I membrane protein; Extracellular side . Cell projection, lamellipodium membrane; Single-pass type I membrane protein; Extracellular side . Cell surface . Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes (By similarity). . |
| Tissue Expression | Detected only in malignant melanoma cells. |
| Signaling_pathway | |
| Research Areas | |
| Function | Function:Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.,miscellaneous:Valuable marker for several incompletely differentiated precursor cells.,PTM:N-glycosylated.,PTM:O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4.,PTM:Phosphorylation by PRKCA regulates its subcellular location and function in cell motility.,similarity:Contains 15 CSPG (NG2) repeats.,similarity:Contains 2 laminin G-like domains.,subcellular location:Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes.,subunit:Interacts with the first PDZ domain of MPDZ. Interacts with PRKCA. Binds TNC, laminin-1, COL5A1 and COL6A2. Interacts with PLG and angiostatin. Binds FGF2 and PDGFA. Interacts with GRIP1, GRIP2 and GRIA2. Forms a ternary complex with GRIP1 and GRIA2 (By similarity). Interacts with LGALS3 and the integrin composed of ITGB1 and ITGA3. Interacts with ITGA4 through its chondroitin sulfate glycosaminoglycan. Interacts with BCAR1, CDC42 and ACK1. Interacts with MMP16.,tissue specificity:Detected only in malignant melanoma cells., |
