Cytokeratin 7 [ABT436] mouse mAb
Ucallm_WM1476-50
Price range: $268.00 through $328.00
Product details
Background:
CK7 is a cytokeratin with a molecular weight of 54 kDa, which mainly marks glandular epithelium and transitional epithelium. It was positive in the epithelium of breast, thyroid, ovary, endometrium and lung, but negative in the epithelium of gastrointestinal tract, colon and prostate. It is mainly used for the study of epithelial tumors. It is often used in combination with CK20 for differential diagnosis of gastrointestinal adenocarcinoma, lung adenocarcinoma and breast cancer, and combined with EMA for diagnosis of synovial sarcoma.
Specifications
| Size | 50μL/100μL |
| Target | Cytokeratin 7 |
| Reactivity | Human |
| Applications | IHC, WB, IF, ELISA |
| MW(Calculated) | 51kD |
| MW(Observed) | 51kD |
| Host Species | Mouse |
| Isotype | IgG1,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1000; WB 1:500-2000; IF 1:100-500; ELISA 1:1000-5000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RMM436 |
| Immunogen | Synthesized peptide derived from human Cytokeratin 7 AA range: 350-469 |
| Specificity | The antibody can specifically recognize human CK7 protein, and shows no cross reaction with CK1, 4, 5, 6, 8, 10, 14, 17, 18, 19, 20. In western blotting of Hela, A431, A549 and Hela cell lysates, the antibody can label a 51 kDa band corresponding to CK7 in A549 and Hela cell lysates. |
| Gene Name | KRT7 SCL |
| Protein Name | CK 7; CK-7; CK7; Cytokeratin 7; Cytokeratin-7; D15Wsu77e; K2C7; K2C7_HUMAN; K7; Keratin 7; Keratin 7, type II; Keratin type II cytoskeletal 7; Keratin, 55K type II cytoskeletal; Keratin, simple epithelial; Keratin, simple epithelial type I, K7; Keratin, type II cytoskeletal 7; Keratin-7; Krt2-7; KRT7; MGC11625; MGC129731; MGC3625; Sarcolectin; SCL; Type II mesothelial keratin K7; Type-II keratin Kb7 |
| Other Name | CK 7; / CK-7; / CK7; / Cytokeratin 7; / CytokeRatin-7; / D15Wsu77e; / K2C7; / K2C7_HUMAN; / K7; / KeRatin 7; / KeRatin 7, type II; / KeRatin type II cytoskeletal 7; / KeRatin, 55K type II cytoskeletal; / KeRatin, simple epithelial; / KeRatin, simple epithelial type I, K7; / KeRatin, type II cytoskeletal 7; / KeRatin-7; / Krt2-7; / KRT7; / MGC11625; / MGC129731; / MGC3625; / Sarcolectin; / SCL; / Type II mesothelial keRatin K7; / Type-II keRatin Kb7 |
| Species | Human |
| Gene ID | 3855 |
| Uniprot | P08729 |
| Species.1 | |
| Gene ID.1 | |
| Uniprot.1 | |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasmic, Membranous |
| Tissue Expression | Pancreas |
| Signaling_pathway | |
| Research Areas | |
| Function | Function:Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).,induction:Up-regulated by retinoic acid.,mass spectrometry: PubMed:11840567,miscellaneous:There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).,PTM:Arg-20 is dimethylated, probably to asymmetric dimethylarginine.,similarity:Belongs to the intermediate filament family.,subunit:Heterotetramer of two type I and two type II keratins. Interacts with eukaryotic translation initiator factor 3 (eIF3) subunit EIF3S10 and with HPV16 E7.,tissue specificity:Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus., |
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