Price range: $268.00 through $328.00
Background:
Dendritic cells (DCs) are the most potent antigen-presenting cells. Immature DCs efficiently capture antigens and differentiate into interdigitating dendritic cells (IDCs) in lymphoid tissues that induce primary T-cell responses (summary by de Saint-Vis et al., 1998 [PubMed 9768752]).[supplied by OMIM, Dec 2010],
| Size | 50μL/100μL |
| Target | DC-LAMP |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 44kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000; IHC 1:50-200; ELISA 1:10000-20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthetic peptide from human protein at AA range: 261-310 |
| Specificity | The antibody detects endogenous DC-LAMP |
| Gene Name | LAMP3 DCLAMP TSC403 |
| Protein Name | Lysosome-associated membrane glycoprotein 3 (LAMP-3) (Lysosomal-associated membrane protein 3) (DC-lysosome-associated membrane glycoprotein) (DC LAMP) (Protein TSC403) (CD antigen CD208) |
| Other Name | Lysosome-associated membrane glycoprotein 3; / LAMP-3; / Lysosomal-associated membrane protein 3; / DC-lysosome-associated membrane glycoprotein; / DC LAMP; / Protein TSC403; / CD antigen CD208 |
| Species | Human |
| Gene ID | 27074 |
| Uniprot | Q9UQV4, |
| Species.1 | Mouse |
| Gene ID.1 | 239739 |
| Uniprot.1 | Q7TST5 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cell surface . Lysosome membrane; Single-pass type I membrane protein . Cytoplasmic vesicle membrane; Single-pass type I membrane protein . Early endosome membrane; Single-pass type I membrane protein . During dendritic cell maturation, detected on cytoplasmic vesicles (the MHC II compartment) that contain MHC II proteins, LAMP1, LAMP2 and LAMP3 (PubMed:9768752). Detected on lysosomes in mature dendritic cells (PubMed:9768752). . |
| Tissue Expression | Detected in tonsil interdigitating dendritic cells, in spleen, lymph node, Peyer's patches in the small instestine, in thymus medulla and in B-cells (at protein level). Expressed in lymphoid organs and dendritic cells. Expressed in lung. Up-regulated in carcinomas of the esophagus, colon, rectum, ureter, stomach, breast, fallopian tube, thyroid and parotid tissues. |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Lysosome |
| Research Areas | >>Lysosome |
| Function | Function:Might change the lysosome function after the transfer of peptide-MHC class II molecules to the surface of dendritic cells.,similarity:Belongs to the LAMP family.,tissue specificity:Expressed in lymphoid organs and dendritic cells. Expressed in lung. Up-regulated in carcinomas of the esophagus, colon, rectum, ureter, stomach, breast, fallopian tube, thyroid and parotid tissues., |
