Price range: $268.00 through $328.00
Background:
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009],
| Size | 50μL/100μL |
| Target | NOS3/eNOS |
| Reactivity | Human |
| Applications | IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 130-140kD |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; ELISA 1:10000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | Affinity purification |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 15F7 |
| Immunogen | Purified recombinant fragment of human NOS3 expressed in E. Coli. |
| Specificity | NOS3 Monoclonal Antibody detects endogenous levels of NOS3 protein. |
| Gene Name | NOS3 |
| Protein Name | Nitric oxide synthase, endothelial |
| Other Name | NOS3; / Nitric oxide synthase; / endothelial; / Constitutive NOS; / cNOS; / EC-NOS; / Endothelial NOS; / eNOS; / NOS type III; / NOSIII |
| Species | Human |
| Gene ID | 4846 |
| Uniprot | P29474, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | P70313 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity. |
| Tissue Expression | Platelets, placenta, liver and kidney. |
| Signaling_pathway | Organismal Systems >> Immune system >> Platelet activation;Organismal Systems >> Endocrine system >> Estrogen signaling pathway;Organismal Systems >> Endocrine system >> Oxytocin signaling pathway;Organismal Systems >> Endocrine system >> Relaxin signaling pathway;Environmental Information Processing >> Signal transduction >> VEGF signaling pathway;Environmental Information Processing >> Signal transduction >> Apelin signaling pathway;Environmental Information Processing >> Signal transduction >> HIF-1 signaling pathway;Environmental Information Processing >> Signal transduction >> Calcium signaling pathway;Environmental Information Processing >> Signal transduction >> Sphingolipid signaling pathway;Environmental Information Processing >> Signal transduction >> cGMP-PKG signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway |
| Research Areas | >>Arginine biosynthesis; / >>Arginine and proline metabolism; / >>Metabolic pathways; / >>Calcium signaling pathway; / >>cGMP-PKG signaling pathway; / >>HIF-1 signaling pathway; / >>Sphingolipid signaling pathway; / >>PI3K-Akt signaling pathway; / >>VEGF signaling pathway; / >>Apelin signaling pathway; / >>Platelet activation; / >>Estrogen signaling pathway; / >>Oxytocin signaling pathway; / >>Relaxin signaling pathway; / >>Insulin resistance; / >>AGE-RAGE signaling pathway in diabetic complications; / >>Diabetic cardiomyopathy; / >>Lipid and atherosclerosis; / >>Fluid shear stress and atherosclerosis |
| Function | Catalytic activity:L-arginine + n NADPH + n H(+) + m O(2) = citrulline + nitric oxide + n NADP(+).,cofactor:Binds 1 FAD.,cofactor:Binds 1 FMN.,cofactor:Heme group.,cofactor:Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.,enzyme regulation:Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.,Function:Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.,online information:Nitric oxide synthase entry,polymorphism:Variation in NOS3 seem to be associated with susceptibility to coronary spasm.,similarity:Belongs to the NOS family.,similarity:Contains 1 FAD-binding FR-type domain.,similarity:Contains 1 flavodoxin-like domain.,subcellular location:Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.,subunit:Homodimer. Interacts with NOSIP and NOSTRIN.,tissue specificity:Platelets, placenta, liver and kidney., |
