Name: U-Blot® Keap1 Rabbit mAb
SKU: WR4257
Price: 268 USD
Availability: InStock

U-Blot® Keap1 Rabbit mAb

Western blot analysis of lysates from HEK293 cells, using Keap1 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.

WR4257 Western blot analysis of lysates from HEK293 cells, using Keap1 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.

Price range: $268.00 through $328.00

Size

100μl

50μl

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SKU: WR4257-50

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Product details

Background:kelch like ECH associated protein 1(KEAP1) Homo sapiens This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq, Jul 2008],

Specifications

Size	50μL/100μL
Target	Keap1
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, ELISA
MW(Calculated)	70kD
MW(Observed)	60-70kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0444R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	KEAP1 INRF2 KIAA0132 KLHL19
Protein Name	Kelch-like ECH-associated protein 1 (Cytosolic inhibitor of Nrf2) (INrf2) (Kelch-like protein 19)
Other Name	KEAP1; / INRF2; / KIAA0132; / KLHL19; / Kelch-like ECH-associated protein 1; / Cytosolic inhibitor of Nrf2; / INrf2; / Kelch-like protein 19
Species	Human
Gene ID	9817
Uniprot	Q14145,
Species.1	Mouse
Gene ID.1	50868
Uniprot.1	Q9Z2X8,
Species.2	Rat
Gene ID.2	117519
Uniprot.2	P57790
Cellular Localization	Cytoplasm, Nucleus
Tissue Expression	Broadly expressed, with highest levels in skeletal muscle.
Signaling_pathway	Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: specific types >> Hepatocellular carcinoma;Human Diseases >> Neurodegenerative disease >> Parkinson disease
Research Areas	>>Ubiquitin mediated proteolysis; / >>Parkinson disease; / >>Pathways in cancer; / >>Chemical carcinogenesis - reactive oxygen species; / >>Hepatocellular carcinoma; / >>Fluid shear stress and atherosclerosis
Function	Disease:Defects in KEAP1 may be a cause of breast cancer.,Disease:Defects in KEAP1 may be involved in non small cell lung carcinomas (NSCLC) and lung adenocarcinoma.,Domain:The Kelch repeats mediate interaction with NF2L2/NRF2, BPTF and PGAM5.,enzyme regulation:Ubiquitination and subsequent degradation of PGAM5 is inhibited by oxidative stress and sulforaphane.,Function:Retains NFE2L2/NRF2 in the cytosol. Functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. May also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.,PTM:Ubiquitinated and subject to proteasomal degradation.,similarity:Contains 1 BACK (BTB/Kelch associated) domain.,similarity:Contains 1 BTB (POZ) domain.,similarity:Contains 6 Kelch repeats.,subcellular location:Shuttles between cytoplasm and nucleus.,subunit:Homodimer. Interacts with the N-terminal regulatory domain of NF2L2/NRF2. Interacts with BPTF and PTMA. Interacts with CUL3. Part of a complex that contains KEAP1, CUL3 and RBX1. Interacts with PGAM5.,tissue specificity:Broadly expressed, with highest levels in skeletal muscle.,

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U-Blot® Keap1 Rabbit mAb

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U-Blot® Keap1 Rabbit mAb

Product details

Specifications

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