MELK Polyclonal Antibody
Ucallm_WP13143-50
Price range: $268.00 through $328.00
Product details
Background:
catalytic activity:ATP + a protein = ADP + a phosphoprotein., function:Phosphorylates ZNF622 and may contribute to its redirection to the nucleus. May be involved in the inhibition of spliceosome assembly during mitosis., PTM:Autophosphorylated. Thr-478 phosphorylation during mitosis promotes interaction with PPP1R8., similarity:Belongs to the protein kinase superfamily., similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily., similarity:Contains 1 KA1 (kinase-associated) domain., similarity:Contains 1 protein kinase domain., subunit:Interacts with ZNF622 and PPP1R8., tissue specificity:Expressed in placenta, kidney, thymus, testis, ovary and intestine.,
Specifications
| Size | 50μL/100μL |
| Target | MELK |
| Reactivity | Human, Mouse |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 75kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MELK. AA range:431-480 |
| Specificity | MELK Polyclonal Antibody detects endogenous levels of MELK protein. |
| Gene Name | MELK |
| Protein Name | Maternal embryonic leucine zipper kinase |
| Other Name | MELK; / KIAA0175; / Maternal embryonic leucine zipper kinase; / hMELK; / Protein kinase Eg3; / pEg3 kinase; / Protein kinase PK38; / hPK38; / Tyrosine-protein kinase MELK |
| Species | Human |
| Gene ID | 9833 |
| Uniprot | Q14680, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q61846 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cell membrane; Peripheral membrane protein . |
| Tissue Expression | Expressed in placenta, kidney, thymus, testis, ovary and intestine. |
| Signaling_pathway | |
| Research Areas | |
| Function | Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,Function:Phosphorylates ZNF622 and may contribute to its redirection to the nucleus. May be involved in the inhibition of spliceosome assembly during mitosis.,PTM:Autophosphorylated. Thr-478 phosphorylation during mitosis promotes interaction with PPP1R8.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.,similarity:Contains 1 KA1 (kinase-associated) domain.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with ZNF622 and PPP1R8.,tissue specificity:Expressed in placenta, kidney, thymus, testis, ovary and intestine., |
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