Name: MMP-1 Monoclonal Antibody
SKU: WM0322
Price: 268 USD
Availability: InStock

Product details

Background:
matrix metallopeptidase 1(MMP1) Homo sapiens This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This secreted protease breaks down the interstitial collagens, including types I, II, and III. The gene is part of a cluster of MMP genes on chromosome 11. Mutations in this gene are associated with chronic obstructive pulmonary disease (COPD). Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016],

Specifications

Size	50μL/100μL
Target	MMP-1
Reactivity	Human
Applications	WB, IHC, IF, FC, ELISA
MW(Calculated)	54kD
MW(Observed)
Host Species	Mouse
Isotype
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:500-1:2000; IHC 1:200-1:1000; IF 1:200-1:1000; Flow Cyt 1:200-1:400; ELISA 1:10000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	Affinity purification
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	12F9
Immunogen	Purified recombinant fragment of human MMP-1 expressed in E. Coli.
Specificity	MMP-1 Monoclonal Antibody detects endogenous levels of MMP-1 protein.
Gene Name	MMP1
Protein Name	Interstitial collagenase
Other Name	MMP1; / CLG; / Interstitial collagenase; / Fibroblast collagenase; / Matrix metalloproteinase-1; / MMP-1
Species	Human
Gene ID	4312
Uniprot	P03956
Species.1
Gene ID.1
Uniprot.1
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Secreted, extracellular space, extracellular matrix .
Tissue Expression	Fibroblast,Ovary,Synovial cell,Synovial membrane,Thyroid,
Signaling_pathway	Organismal Systems >> Immune system >> IL-17 signaling pathway;Organismal Systems >> Endocrine system >> PPAR signaling pathway;Organismal Systems >> Endocrine system >> Relaxin signaling pathway;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: specific types >> Bladder cancer;Human Diseases >> Immune disease >> Rheumatoid arthritis
Research Areas	>>PPAR signaling pathway; / >>IL-17 signaling pathway; / >>Relaxin signaling pathway; / >>Coronavirus disease - COVID-19; / >>Pathways in cancer; / >>Bladder cancer; / >>Rheumatoid arthritis; / >>Lipid and atherosclerosis
Function	Catalytic activity:Cleavage of the triple helix of collagen at about three-quarters of the length of the molecule from the N-terminus, at 775-Gly-\|-Ile-776 in the alpha-1(I) chain. Cleaves synthetic substrates and alpha-macroglobulins at bonds where P1' is a hydrophobic residue.,cofactor:Binds 2 zinc ions per subunit.,cofactor:Binds 4 calcium ions per subunit.,Domain:The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.,Domain:There are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).,enzyme regulation:Can be activated without removal of the activation peptide.,Function:Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.,online information:Collagenase entry,PTM:Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.,similarity:Belongs to the peptidase M10A family.,similarity:Contains 4 hemopexin-like domains.,subunit:Interacts with HIV-1 Tat.,