SKU: WR4269 Categories: ,

U-Blot® Perilipin-1 Rabbit mAb

Price range: $268.00 through $328.00

SKU: WR4269-50
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Product details

Background:The protein encoded by this gene coats lipid storage droplets in adipocytes, thereby protecting them until they can be broken down by hormone-sensitive lipase. The encoded protein is the major cAMP-dependent protein kinase substrate in adipocytes and, when unphosphorylated, may play a role in the inhibition of lipolysis. Alternatively spliced transcript variants varying in the 5′ UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq, Feb 2009]

Specifications

Size50μL/100μL
TargetPLIN1
ReactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF, IP, ELISA
MW(Calculated)56kD
MW(Observed)56kD
Host SpeciesRabbit
IsotypeIgG,Kappa
Conjugate/ModificationUnmodified
Recommended Dilution RatioIHC 1:400-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationPBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
PurificationRecombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
ClonalityMonoclonal
Clone NumberRM0458R
ImmunogenThe specific immunogen used to produce this antibody is proprietary information.
SpecificityEndogenous
Gene NamePLIN1 PERI PLIN
Protein NamePerilipin-1 (Lipid droplet-associated protein)
Other Name
SpeciesHuman
Gene ID5346
UniprotO60240,
Species.1Mouse
Gene ID.1103968
Uniprot.1Q8CGN5,
Species.2Rat
Gene ID.225629
Uniprot.2P43884
Cellular LocalizationEndoplasmic reticulum
Tissue ExpressionDetected in adipocytes from white adipose tissue (at protein level) (PubMed:27832861). Detected in visceral adipose tissue and mammary gland (PubMed:9521880).
Signaling_pathwayOrganismal Systems >> Endocrine system >> PPAR signaling pathway;Environmental Information Processing >> Signal transduction >> Apelin signaling pathway
Research Areas
FunctionModulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness. Plays a role in unilocular lipid droplet formation by activating CIDEC. Their interaction promotes lipid droplet enlargement and directional net neutral lipid transfer. May modulate lipolysis and triglyceride levels.
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