Price range: $268.00 through $328.00
Background:PRKD1 is a serine/threonine kinase that regulates a variety of cellular functions, including membrane receptor signaling, transport at the Golgi, protection from oxidative stress at the mitochondria, gene transcription, and regulation of cell shape, motility, and adhesion (summary by Eiseler et al., 2009 [PubMed 19329994]).[supplied by OMIM, Nov 2010],
| Size | 50μL/100μL |
| Target | PKD1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 102kD |
| MW(Observed) | 102kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1047R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | PRKD1 |
| Protein Name | Serine/threonine-protein kinase D1 |
| Other Name | PRKD1; / PKD; / PKD1; / PRKCM; / Serine/threonine-protein kinase D1; / Protein kinase C mu type; / Protein kinase D; / nPKC-D1; / nPKC-mu |
| Species | Human |
| Gene ID | 5587 |
| Uniprot | Q15139, |
| Species.1 | Mouse |
| Gene ID.1 | 18760 |
| Uniprot.1 | Q62101, |
| Species.2 | Rat |
| Gene ID.2 | 85421 |
| Uniprot.2 | Q9WTQ1 |
| Cellular Localization | Cytoplasm . Cell membrane . Golgi apparatus, trans-Golgi network . Translocation to the cell membrane is required for kinase activation. |
| Tissue Expression | Placenta,Testis, |
| Signaling_pathway | Organismal Systems >> Endocrine system >> Aldosterone synthesis and secretion;Environmental Information Processing >> Signal transduction >> Rap1 signaling pathway |
| Research Areas | >>Rap1 signaling pathway; / >>Aldosterone synthesis and secretion; / >>Chemical carcinogenesis - reactive oxygen species |
| Function | Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Activated by diacylglycerol and phorbol esters.,Function:Calcium-independent, phospholipid-dependent, serine- and threonine-specific kinase involved in resistance to oxidative stress.,PTM:Phosphorylation of Ser-738 and/or Ser-742 in activated PKD is mediated by transphosphorylation (By similarity). Phosphorylation of Tyr-463 mediated by the Src/Abl pathway in response to oxidative stress activates the kinase.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers.,subunit:Interacts (via N-terminus) with ADAP1/CENTA1. Interacts with Src., |
