Name: U-Blot® PPARα Rabbit mAb
SKU: WR4210
Price: 268 USD
Availability: InStock

U-Blot® PPARα Rabbit mAb

Western blot analysis of lysates from HepG2 cells, using PPARα Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.

WR4210 Western blot analysis of lysates from HepG2 cells, using PPARα Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.

Price range: $268.00 through $328.00

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100μl

50μl

Clear

SKU: WR4210-50

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Product details

Background:peroxisome proliferator activated receptor alpha(PPARA) Homo sapiens Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for thi

Specifications

Size	50μL/100μL
Target	PPAR α
Reactivity	Human, Mouse, Rat
Applications	WB, ELISA, IHC
MW(Calculated)	52kD
MW(Observed)	52kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:1000-1:5000; ELISA 1:5000-1:20000; IHC 1:200-1:1000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0384R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	PPARA NR1C1 PPAR
Protein Name	Peroxisome proliferator-activated receptor alpha
Other Name	PPARA; / NR1C1; / PPAR; / Peroxisome proliferator-activated receptor alpha; / PPAR-alpha; / Nuclear receptor subfamily 1 group C member 1
Species	Human
Gene ID	5465
Uniprot	Q07869,
Species.1	Mouse
Gene ID.1	19013
Uniprot.1	P23204,
Species.2	Rat
Gene ID.2	25747
Uniprot.2	P37230
Cellular Localization	Nucleus
Tissue Expression	Skeletal muscle, liver, heart and kidney. Expressed in monocytes (PubMed:28167758).
Signaling_pathway	Organismal Systems >> Endocrine system >> Glucagon signaling pathway;Organismal Systems >> Endocrine system >> Adipocytokine signaling pathway;Organismal Systems >> Endocrine system >> PPAR signaling pathway;Environmental Information Processing >> Signal transduction >> cAMP signaling pathway
Research Areas	>>PPAR signaling pathway; / >>cAMP signaling pathway; / >>Adipocytokine signaling pathway; / >>Glucagon signaling pathway; / >>Insulin resistance; / >>Non-alcoholic fatty liver disease; / >>Alcoholic liver disease; / >>Hepatitis C; / >>Chemical carcinogenesis - receptor activation; / >>Diabetic cardiomyopathy
Function	Function:Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids.,online information:Peroxisome proliferator-activated receptor entry,similarity:Belongs to the nuclear hormone receptor family. NR1 subfamily.,similarity:Contains 1 nuclear receptor DNA-binding domain.,subunit:Heterodimer with the retinoid X receptor. Interacts with NCOA3 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Also interacts with PPARBP coactivator in vitro. Interacts with AKAP13.,tissue specificity:Skeletal muscle, liver, heart and kidney.,