Name: U-Blot® PRKACA Rabbit mAb
SKU: WR4252
Price: 268 USD
Availability: InStock

Product details

Background:This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Altern

Specifications

Size	50μL/100μL
Target	PKACA
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	40kD
MW(Observed)	40kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:2000-1:10000; WB 1:1000-1:5000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0438R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	PRKACA
Protein Name	cAMP-dependent protein kinase catalytic subunit alpha
Other Name	PRKACA; / PKACA; / cAMP-dependent protein kinase catalytic subunit alpha; / PKA C-alpha
Species	Human
Gene ID	5566
Uniprot	P17612,
Species.1	Mouse
Gene ID.1	18747
Uniprot.1	P05132,
Species.2	Rat
Gene ID.2
Uniprot.2	P27791
Cellular Localization	Cytoplasm, Membrane
Tissue Expression	Isoform 1 is ubiquitous. Isoform 2 is sperm-specific and is enriched in pachytene spermatocytes but is not detected in round spermatids.
Signaling_pathway	Cellular Processes >> Transport and catabolism >> Autophagy - animal;Cellular Processes >> Cellular community - eukaryotes >> Tight junction;Cellular Processes >> Cellular community - eukaryotes >> Gap junction;Organismal Systems >> Immune system >> Platelet activation;Organismal Systems >> Immune system >> Chemokine signaling pathway;Organismal Systems >> Endocrine system >> Insulin secretion;Organismal Systems >> Endocrine system >> Insulin signaling pathway;Organismal Systems >> Endocrine system >> Glucagon signaling pathway;Organismal Systems >> Endocrine system >> GnRH signaling pathway;Organismal Systems >> Endocrine system >> Estrogen signaling pathway;Organismal Systems >> Endocrine system >> Oxytocin signaling pathway;Organismal Systems >> Endocrine system >> Relaxin signaling pathway;Organismal Systems >> Endocrine system >> Growth hormone synthesis, secretion and action;Organismal Systems >> Endocrine system >> Thyroid hormone signaling pathway;Organismal Systems >> Endocrine system >> Aldosterone synthesis and secretion;Organismal Systems >> Circulatory system >> Adrenergic signaling in cardiomyocytes;Organismal Systems >> Circulatory system >> Vascular smooth muscle contraction;Organismal Systems >> Nervous system >> Glutamatergic synapse;Organismal Systems >> Nervous system >> Cholinergic synapse;Organismal Systems >> Nervous system >> Dopaminergic synapse;Organismal Systems >> Sensory system >> Inflammatory mediator regulation of TRP channels;Organismal Systems >> Aging >> Longevity regulating pathway;Organismal Systems >> Aging >> Longevity regulating pathway - multiple species;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Neurodegenerative disease >> Parkinson disease;Human Diseases >> Neurodegenerative disease >> Prion disease;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> Ras signaling pathway;Environmental Information Processing >> Signal transduction >> Wnt signaling pathway;Environmental Information Processing >> Signal transduction >> Hedgehog signaling pathway;Environmental Information Processing >> Signal transduction >> Apelin signaling pathway;Environmental Information Processing >> Signal transduction >> Calcium signaling pathway;Environmental Information Processing >> Signal transduction >> cAMP signaling pathway
Research Areas	>>Endocrine resistance; / >>MAPK signaling pathway; / >>Ras signaling pathway; / >>Calcium signaling pathway; / >>cAMP signaling pathway; / >>Chemokine signaling pathway; / >>Oocyte meiosis; / >>Autophagy - animal; / >>Longevity regulating pathway; / >>Longevity regulating pathway - multiple species; / >>Adrenergic signaling in cardiomyocytes; / >>Vascular smooth muscle contraction; / >>Wnt signaling pathway; / >>Hedgehog signaling pathway; / >>Apelin signaling pathway; / >>Tight junction; / >>Gap junction; / >>Platelet activation; / >>Circadian entrainment; / >>Thermogenesis; / >>Long-term potentiation; / >>Retrograde endocannabinoid signaling; / >>Glutamatergic synapse; / >>Cholinergic synapse; / >>Serotonergic synapse; / >>GABAergic synapse; / >>Dopaminergic synapse; / >>Olfactory transduction; / >>Taste transduction; / >>Inflammatory mediator regulation of TRP channels; / >>Insulin signaling pathway; / >>Insulin secretion; / >>GnRH signaling pathway; / >>Ovarian steroidogenesis; / >>Progesterone-mediated oocyte maturation; / >>Estrogen signaling pathway; / >>Melanogenesis; / >>Thyroid hormone synthesis; / >>Thyroid hormone signaling pathway; / >>Oxytocin signaling pathway; / >>Glucagon signaling pathway; / >>Regulation of lipolysis in adipocytes; / >>Renin secretion; / >>Aldosterone synthesis and secretion; / >>Relaxin signaling pathway; / >>Cortisol synthesis and secretion; / >>Parathyroid hormone synthesis, secretion and action; / >>Cushing syndrome; / >>Growth hormone synthesis, secretion and action; / >>Endocrine and other factor-regulated calcium reabsorption; / >>Vasopressin-regulated water reabsorption; / >>Salivary secretion; / >>Gastric acid secretion; / >>Bile secretion; / >>Parkinson disease; / >>Prion disease; / >>Cocaine addiction; / >>Amphetamine addiction; / >>Morphine addiction; / >>Alcoholism; / >>Vibrio cholerae infection; / >>Amoebiasis; / >>Human cytomegalovirus infection; / >>Human papillomavirus infection; / >>Human T-cell leukemia virus 1 infection; / >>Pathways in cancer; / >>Viral carcinogenesis; / >>Proteoglycans in cancer; / >>Chemical carcinogenesis - receptor activation; / >>Dilated cardiomyopathy
Function	Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Activated by cAMP.,Function:Phosphorylates a large number of substrates in the cytoplasm and the nucleus.,PTM:Asn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 protein kinase domain.,subcellular location:Translocates into the nucleus (monomeric catalytic subunit) (By similarity). The inactive holoenzyme is found in the cytoplasm.,subunit:A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits.,tissue specificity:Isoform 2 is sperm specific.,