WB 1:500-2000; IHC 1:50-300 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
1 mg/ml
Clonality
Polyclonal
Clone Number
Immunogen
Synthesized peptide derived from human RBM25 AA range: 763-813
Specificity
This antibody detects endogenous levels of RBM25 at Human/Mouse
Gene Name
RBM25 RNPC7
Protein Name
RBM25
Other Name
Species
Human
Gene ID
58517
Uniprot
P49756,
Species.1
Mouse
Gene ID.1
67039
Uniprot.1
B2RY56
Species.2
Gene ID.2
Uniprot.2
Cellular Localization
Nucleus speckle. Cytoplasm. Colocalizes predominantly, with SFRS2 and LUC7L3 splicing factors, in nuclear speckles. Cytoplasmic localization is faint.
Tissue Expression
Signaling_pathway
Research Areas
>>Spliceosome
Function
PTM:Sumoylated.,sequence Caution:Contaminating sequence. Potential poly-A sequence.,sequence Caution:Translation N-terminally extended.,similarity:Contains 1 PWI domain.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subcellular location:Detected predominantly in nuclear speckles. Cytoplasmic localization is faint.,subunit:Interacts with SRRM1. Specifically associates with functional splicing complexes, including Sm proteins and U1, U2, U4, U5 and U6 snRNAs. Associates with exon junction complex (EJC) proteins, including APEX1, BAT1, NCBP1, RBM8A and RNPS1. Interaction with NCBP1 is RNA-dependent.,