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Home/Reagents/Antibody/Primary antibody/Polyclonal Antibody/Rabbit pAb/Sp1 Polyclonal Antibody
SKU: WP14547 Categories: ,

Sp1 Polyclonal Antibody

Ucallm_WP14547-50

Price range: $268.00 through $328.00

SKU: WP14547-100
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Product details

Background:

The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014],

Specifications

Size50μL/100μL
TargetSp1
ReactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)85kD
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from human SP1. AA range:706-755
SpecificitySP1 Polyclonal Antibody detects endogenous levels of SP1 protein.
Gene NameSP1
Protein NameTranscription factor Sp1
Other NameSP1; / TSFP1; / Transcription factor Sp1
SpeciesHuman
Gene ID6667
UniprotP08047,
Species.1Mouse
Gene ID.120683
Uniprot.1O89090,
Species.2Rat
Gene ID.224790
Uniprot.2Q01714
Cellular LocalizationNucleus. Cytoplasm. Nuclear location is governed by glycosylated/phosphorylated states. Insulin promotes nuclear location, while glucagon favors cytoplasmic location.
Tissue ExpressionUp-regulated in adenocarcinomas of the stomach (at protein level). Isoform 3 is ubiquitously expressed at low levels.
Signaling_pathwayCellular Processes >> Transport and catabolism >> Mitophagy - animal;Organismal Systems >> Endocrine system >> Estrogen signaling pathway;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: overview >> Transcriptional misregulation in cancer;Human Diseases >> Cancer: specific types >> Breast cancer;Human Diseases >> Neurodegenerative disease >> Huntington disease;Human Diseases >> Neurodegenerative disease >> Spinocerebellar ataxia;Environmental Information Processing >> Signal transduction >> TGF-beta signaling pathway
Research Areas>>Endocrine resistance; / >>Mitophagy - animal; / >>TGF-beta signaling pathway; / >>Estrogen signaling pathway; / >>Cortisol synthesis and secretion; / >>Parathyroid hormone synthesis, secretion and action; / >>Cushing syndrome; / >>Huntington disease; / >>Spinocerebellar ataxia; / >>Human cytomegalovirus infection; / >>Pathways in cancer; / >>Transcriptional misregulation in cancer; / >>Breast cancer; / >>Choline metabolism in cancer; / >>Diabetic cardiomyopathy
FunctionFunction:Binds to GC box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites. Can interact with G/C-rich motifs from serotonin receptor promoter.,PTM:O-glycosylated; contains N-acetylglucosamine side chains.,similarity:Belongs to the Sp1 C2H2-type zinc-finger protein family.,similarity:Contains 3 C2H2-type zinc fingers.,subunit:Interacts with ATF7IP, ATF7IP2, POGZ, HCFC1, AATF and PHC2. Interacts with varicella-zoster virus IE62 protein and HIV-1 Vpr. Interacts with SV40 VP2/3 proteins. Interacts with SV40 major capsid protein VP1; this interaction leads to a cooperativity between the two proteins in DNA binding.,
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