Price range: $268.00 through $328.00
Background:
The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM, Jul 2010],
| Size | 50μL/100μL |
| Target | Synaptotagmin 1/2 |
| Reactivity | Human |
| Applications | WB, ELISA |
| MW(Calculated) | |
| MW(Observed) | |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; ELISA 1:10000; Not yet tested in other applications. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | Affinity purification |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 20E1 |
| Immunogen | Purified recombinant fragment of Synaptotagmin 1 expressed in E. Coli. |
| Specificity | Synaptotagmin 1 Monoclonal Antibody detects endogenous levels of Synaptotagmin 1 protein. |
| Gene Name | SYT1 |
| Protein Name | Synaptotagmin-1 |
| Other Name | SYT1; / SVP65; / SYT; / Synaptotagmin-1; / Synaptotagmin I; / SytI; / p65 |
| Species | Human |
| Gene ID | 6857 |
| Uniprot | P21579, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | P46096 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasmic vesicle, secretory vesicle membrane; Single-pass membrane protein . Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass membrane protein . Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane; Single-pass membrane protein . Cytoplasm . |
| Tissue Expression | Expressed in melanocytes (PubMed:23999003). |
| Signaling_pathway | Organismal Systems >> Nervous system >> Synaptic vesicle cycle |
| Research Areas | >>Synaptic vesicle cycle |
| Function | cofactor:Binds 3 calcium ions per subunit. The ions are bound to the C2 domains.,Domain:The first C2 domain mediates Ca(2+)-dependent phospholipid binding.,Domain:The second C2 domain mediates interaction with SV2A and STN2.,Function:May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.,similarity:Belongs to the synaptotagmin family.,similarity:Contains 2 C2 domains.,subcellular location:Synaptic vesicles and chromaffin granules.,subunit:Homotetramer (Probable). Interacts with SCAMP5, STN2, SV2A, SV2B, SV2C and RIMS1. Forms a complex with SV2B, syntaxin 1 and SNAP25., |
