Price range: $268.00 through $328.00
Background:
developmental stage:Exclusively expressed in proliferating cells from the transition G1/S until the end of cytokinesis., PTM:Phosphorylated upon DNA damage, probably by ATM or ATR., subcellular location:During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus., tissue specificity:Expressed in lung carcinoma cell lines but not in normal lung tissues.,
| Size | 50μL/100μL |
| Target | TPX2 |
| Reactivity | Human, Mouse |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 86kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human DIL-2. AA range:301-350 |
| Specificity | TPX2 Polyclonal Antibody detects endogenous levels of TPX2 protein. |
| Gene Name | TPX2 |
| Protein Name | Targeting protein for Xklp2 |
| Other Name | TPX2; / C20orf1; / C20orf2; / DIL2; / HCA519; / Targeting protein for Xklp2; / Differentially expressed in cancerous and non-cancerous lung cells 2; / DIL-2; / Hepatocellular carcinoma-associated antigen 519; / Protein fls353; / Restricted expression prolifera |
| Species | Human |
| Gene ID | 22974 |
| Uniprot | Q9ULW0, |
| Species.1 | Mouse |
| Gene ID.1 | 72119 |
| Uniprot.1 | A2APB8 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus . Cytoplasm, cytoskeleton, spindle . Cytoplasm, cytoskeleton, spindle pole . During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus. Is released from the nucleus in apoptotic cells and is detected on apoptotic microtubules. . |
| Tissue Expression | Expressed in lung carcinoma cell lines but not in normal lung tissues. |
| Signaling_pathway | |
| Research Areas | |
| Function | developmental stage:Exclusively expressed in proliferating cells from the transition G1/S until the end of cytokinesis.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,subcellular location:During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus.,tissue specificity:Expressed in lung carcinoma cell lines but not in normal lung tissues., |
