Price range: $268.00 through $328.00
Background:
This gene encodes a zinc-finger transcriptional regulating protein which interacts with CBP/p300 to regulate the human gene CYP11A1. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014],
| Size | 50μL/100μL |
| Target | TReP-132 |
| Reactivity | Human, Mouse |
| Applications | IHC, IF, ELISA |
| MW(Calculated) | 132kD |
| MW(Observed) | |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:100-1:300; ELISA 1:5000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human TREF1. AA range:1071-1120 |
| Specificity | TReP-132 Polyclonal Antibody detects endogenous levels of TReP-132 protein. |
| Gene Name | TRERF1 |
| Protein Name | Transcriptional-regulating factor 1 |
| Other Name | TRERF1; / BCAR2; / RAPA; / TREP132; / Transcriptional-regulating factor 1; / Breast cancer anti-estrogen resistance 2; / Transcriptional-regulating protein 132; / Zinc finger protein rapa; / Zinc finger transcription factor TReP-132 |
| Species | Human |
| Gene ID | 55809 |
| Uniprot | Q96PN7, |
| Species.1 | Mouse |
| Gene ID.1 | 224829 |
| Uniprot.1 | Q8BXJ2 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus . |
| Tissue Expression | Highest expression was seen in thymus, testis and adrenal cortex, expressed also in the adrenal medulla, thyroid, and stomach. Highly expressed in steroidogenic JEG-3 and MCF-7 cells, low expression was seen in non-steroidogenic Hep-G2 and HEK293 cells. |
| Signaling_pathway | |
| Research Areas | |
| Function | Function:Activates transcription of CYP11A1. Interaction with CREBBP and EP300 results in a synergistic transcriptional activation of CYP11A1.,similarity:Contains 1 ELM2 domain.,similarity:Contains 1 SANT domain.,similarity:Contains 3 C2H2-type zinc fingers.,subunit:Interacts with CREBBP and EP300.,tissue specificity:Highest expression was seen in thymus, testis and adrenal cortex, expressed also in the adrenal medulla, thyroid, and stomach. Highly expressed in steroidogenic JEG-3 and MCF-7 cells, low expression was seen in non-steroidogenic HepG2 and HK293 cells., |
