Name: U-Blot® HBEGF Rabbit mAb
SKU: WR5195
Price: 268 USD
Availability: InStock

U-Blot® HBEGF Rabbit mAb

Ucallm_WR5195-50

Price range: $268.00 through $328.00

Size

100μl

50μl

Clear

SKU: WR5195-100

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Product details

Background:function:May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts and smooth muscle but not endothelial cells. It is able to bind EGF receptors with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.,PTM:O-linked glycan attachment sites were determined by Edman degradation, O-glycanase digest suggests mucin-type glycosylation (done in HB-EGF purified from histiocytic lymphoma cell line U-937).,PTM:Several N-termini have been identified by direct sequencing. The forms with N-termini 63, 73 and 74 have been tested and found to be biologically active.,similarity:Contains 1 EGF-like domain.,subcellular location:Mature HB-EGF is released into the extracellular space and probably binds to a receptor.,

Specifications

Size	50μL/100μL
Target	HBEGF
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, ELISA
MW(Calculated)	23kD
MW(Observed)	21kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM1429R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	HBEGF DTR DTS HEGFL
Protein Name	Proheparin-binding EGF-like growth factor [Cleaved into: Heparin-binding EGF-like growth factor (HB-EGF) (HBEGF) (Diphtheria toxin receptor) (DT-R)]
Other Name
Species	Human
Gene ID	1839
Uniprot	Q99075,
Species.1	Mouse
Gene ID.1
Uniprot.1	Q06186,
Species.2	Rat
Gene ID.2
Uniprot.2	Q06175
Cellular Localization	[Heparin-binding EGF-like growth factor]: Secreted, extracellular space. Mature HB-EGF is released into the extracellular space and probably binds to a receptor.; [Proheparin-binding EGF-like growth factor]: Cell membrane; Single-pass type I membrane protein.
Tissue Expression	Brain,Eye,Histiocytic lymphoma,Macrophage,
Signaling_pathway	Organismal Systems >> Endocrine system >> GnRH signaling pathway;Organismal Systems >> Endocrine system >> Estrogen signaling pathway;Human Diseases >> Cancer: specific types >> Bladder cancer;Environmental Information Processing >> Signal transduction >> ErbB signaling pathway
Research Areas	>>Endocrine resistance; / >>ErbB signaling pathway; / >>GnRH signaling pathway; / >>Estrogen signaling pathway; / >>Parathyroid hormone synthesis, secretion and action; / >>Epithelial cell signaling in Helicobacter pylori infection; / >>Coronavirus disease - COVID-19; / >>Proteoglycans in cancer; / >>Bladder cancer
Function	Function:May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts and smooth muscle but not endothelial cells. It is able to bind EGF receptors with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.,PTM:O-linked glycan attachment sites were determined by Edman degradation, O-glycanase digest suggests mucin-type glycosylation (done in HB-EGF purified from histiocytic lymphoma cell line U-937).,PTM:Several N-termini have been identified by direct sequencing. The forms with N-termini 63, 73 and 74 have been tested and found to be biologically active.,similarity:Contains 1 EGF-like domain.,subcellular location:Mature HB-EGF is released into the extracellular space and probably binds to a receptor.,