U-Blot® Pan-Tri-Methyllysine Rabbit pAb, Biotin
Ucallm_WT9197C-100
$999.00
Product details
This rabbit polyclonal antibody is raised with a chemically methylated protein antigen. The specific anti-tri-methyl-lysine antibody was affinity purified with trimethylated lysine antibodies and affinity adsorbed with N-(epsilon) mono- and di-methylated lysine. The purified anti-trimethyl-lysine antibody was labelled with dbiotin. The antibody could be utilized for detection and localization of proteins with N-trimethylated lysine residues.
Specifications
| Target | Tri-Methyllysine |
| Reactivity | Human, mouse |
| Application | ELISA、 WB、 IF、 IHC |
| MW(Calculated) | Multiple |
| MW(Observed) | Multiple |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate | dBiotin-NHS ester; Biotin/Ab molar ratio - >20:1 |
| Modification | Lysine Trimethylation |
| Modified site | Lys（K） |
| Recommended dilution ratio | |
| Composition | 250 µg/mL in PBS, 50% glycerol |
| Purification process | The antibody was purified on N (epsilon) tri-methyl lysine agarose and absorbed by mono- and di-methyl lysine. |
| Purity | |
| Storage | Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. |
| Stability | Expiration date is one year from date of shipping if stored properly. |
| Clonal | Polyclonal |
| Clone Number | |
| Immunogen | Methylated KLH conjugates |
| Sequence | |
| Specificity | |
| Gene name | |
| Protein Name | |
| Alternative name | Kme3 |
| Organism-1 | |
| GeneID-1 | |
| SwissProt-1 | |
| Organism-2 | |
| GeneID-2 | |
| SwissProt-2 | |
| Organism-3 | |
| GeneID-3 | |
| SwissProt-3 | |
| Organism-4 | |
| GeneID-4 | |
| SwissProt-4 | |
| Background | Histones undergo a wide range of enzyme-catalyzed post-translational modifications, including acetylation, methylation, phosphorylation and ubiquitination. Lysine trimethylation is a vital epigenetic modification that predominantly occurs on histone lysine residues. It is dynamically regulated by histone methyltransferases and demethylases, and extensively distributed in eukaryotic chromatin.Lysine trimethylation participates in governing chromatin structure remodeling, transcriptional activation or repression, cell cycle regulation, and DNA damage repair. Abnormal trimethylation levels are closely associated with tumorigenesis, developmental disorders, inflammation and neurodegenerative diseases, acting as a pivotal molecular marker for epigenetic research and disease mechanism investigation. |
| Cellular Localization |
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