U-Blot® PU.1 Rabbit mAb
Ucallm_WR5293-50
Price range: $268.00 through $328.00
Product details
Background:This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | PU.1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 31kD |
| MW(Observed) | 42kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0951R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | SPI1 |
| Protein Name | Transcription factor PU.1 |
| Other Name | SPI1; / Transcription factor PU.1; / 31 kDa-transforming protein |
| Species | Human |
| Gene ID | 6688 |
| Uniprot | P17947, |
| Species.1 | Mouse |
| Gene ID.1 | 20375 |
| Uniprot.1 | P17433, |
| Species.2 | Rat |
| Gene ID.2 | 366126 |
| Uniprot.2 | Q6BDS1 |
| Cellular Localization | Nucleus . |
| Tissue Expression | In the bone marrow, concentrated in hematopoietic stem cell, lymphoid progenitor, myeloid lineage (granulocyte macrophage progenitors, classical dendritic cells, monocytes) and B-cell clusters. Among B-cells, predominantly expressed in pre-B1 cells (PubMed:33951726). Expressed in germinal center B-cells (PubMed:23166356). |
| Signaling_pathway | Organismal Systems >> Development and regeneration >> Osteoclast differentiation;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: overview >> Transcriptional misregulation in cancer;Human Diseases >> Cancer: specific types >> Acute myeloid leukemia |
| Research Areas | >>Osteoclast differentiation; / >>Human T-cell leukemia virus 1 infection; / >>Pathways in cancer; / >>Transcriptional misregulation in cancer; / >>Acute myeloid leukemia |
| Function | Function:Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing.,induction:Highly expressed in both FV-P and FV-A-induced erythro-leukemia cell lines that have undergone rearrangements of the Spi-1 gene due to the insertion of SFFV.,similarity:Belongs to the ETS family.,similarity:Contains 1 ETS DNA-binding domain.,subunit:Binds DNA as a monomer. Interacts with RUNX1 and SPIB. Interacts with CEBPD and NONO., |
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