U-Blot® RAP1A/RAP1B Rabbit mAb
Ucallm_WR5982-50
Price range: $268.00 through $328.00
Product details
Background：
This gene encodes a member of the Ras family of small GTPases. The encoded protein undergoes a change in conformational state and activity , depending on whether it is bound to GTP or GDP. This protein is activated by several types of guanine nucleotide exchange factors (GEFs) , and inactivated by two groups of GTPase-activating proteins (GAPs) . The activation status of the encoded protein is therefore affected by the balance of intracellular levels of GEFs and GAPs. The encoded protein regulates signaling pathways that affect cell proliferation and adhesion , and may play a role in tumor malignancy. Pseudogenes of this gene have been defined on chromosomes 14 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq , May 2014] ,
Specifications
| Size | 50μL/100μL |
| Target | RAP1A/RAP1B |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 21kD |
| MW(Observed) | 21kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1988R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | RAP1A KREV1 RAP1B |
| Protein Name | Ras-related protein Rap-1A (C21KG) (G-22K) (GTP-binding protein smg p21A) (Ras-related protein Krev-1) Ras-related protein Rap-1b GTP-binding protein smg p21B |
| Other Name | |
| Species | Human |
| Gene ID | 5906;5908 |
| Uniprot | P62834;P61224, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | P62835, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | P62836 |
| Cellular Localization | Cell membrane; Lipid-anchor . Cytoplasm . Cytoplasm, perinuclear region . Cell junction . Early endosome . Recruited from early endosome to late endosome compartment after nerve growth factor (NGF) stimulation. Localized with RAPGEF2 at cell-cell junctions (By similarity). Colocalized with RAPGEF2 in the perinuclear region. . |
| Tissue Expression | Brain,Platelet,Skin,Uterus, |
| Signaling_pathway | Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion;Cellular Processes >> Cellular community - eukaryotes >> Adherens junction;Cellular Processes >> Cellular community - eukaryotes >> Tight junction;Organismal Systems >> Immune system >> Platelet activation;Organismal Systems >> Immune system >> Leukocyte transendothelial migration;Organismal Systems >> Immune system >> Chemokine signaling pathway;Organismal Systems >> Nervous system >> Neurotrophin signaling pathway;Human Diseases >> Cancer: specific types >> Renal cell carcinoma;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> Ras signaling pathway;Environmental Information Processing >> Signal transduction >> Rap1 signaling pathway;Environmental Information Processing >> Signal transduction >> cAMP signaling pathway |
| Research Areas | >>MAPK signaling pathway; / >>Ras signaling pathway; / >>Rap1 signaling pathway; / >>cAMP signaling pathway; / >>Chemokine signaling pathway; / >>Focal adhesion; / >>Tight junction; / >>Platelet activation; / >>Leukocyte transendothelial migration; / >>Long-term potentiation; / >>Neurotrophin signaling pathway; / >>Cushing syndrome; / >>Pancreatic secretion; / >>Renal cell carcinoma; / >>Lipid and atherosclerosis |
| Function | enzyme regulation:Activated by guanine nucleotide-exchange factors (GEF) EPAC and EPAC2 in a cAMP-dependent manner, and GFR.,Function:Induces morphological reversion of a cell line transformed by a Ras oncogene. Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner.,similarity:Belongs to the small GTPase superfamily. Ras family.,subunit:In its GTP-bound form interacts with PLCE1 and RADIL. Interacts with SGSM1, SGSM2 and SGSM3., |
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