Price range: $268.00 through $328.00
Background:
ubiquitin interaction motif containing 1(UIMC1) Homo sapiens This gene encodes a nuclear protein that interacts with Brca1 (breast cancer 1) in a complex to recognize and repair DNA lesions. This protein binds ubiquitinated lysine 63 of histone H2A and H2AX. This protein may also function as a repressor of transcription. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015],
| Size | 50μL/100μL |
| Target | UIMC1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, ELISA |
| MW(Calculated) | |
| MW(Observed) | 79kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000; ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% glycerol,0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from part region of human protein |
| Specificity | UIMC1 Polyclonal Antibody detects endogenous levels of protein. |
| Gene Name | UIMC1 RAP80 RXRIP110 |
| Protein Name | BRCA1-A complex subunit RAP80 (Receptor-associated protein 80) (Retinoid X receptor-interacting protein 110) (Ubiquitin interaction motif-containing protein 1) |
| Other Name | |
| Species | Human |
| Gene ID | 51720 |
| Uniprot | Q96RL1, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q5U5Q9, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | Q5PQK4 |
| Cellular Localization | Nucleus . Localizes at sites of DNA damage at double-strand breaks (DSBs). |
| Tissue Expression | Expressed in testis, ovary, thymus and heart. Expressed in germ cells of the testis. |
| Signaling_pathway | |
| Research Areas | >>Homologous recombination |
| Function | Domain:The Abraxas-interacting region (AIR) mediates the interaction with FAM175A/Abraxas.,Domain:The UIM-linker region between the 2 UIM repeats determines the selectivity for 'Lys-63'-linked ubiquitin. The length of the linker is important. The linker reduces the flexibility between the UIM repeats and promotes high-affinity and linkage-selective interactions.,Function:Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly Act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1.,PTM:Phosphorylated upon DNA damage by ATM or ATR.,PTM:Sumoylated.,sequence Caution:Translated as Glu.,similarity:Belongs to the RAP80 family.,similarity:Contains 2 UIM (ubiquitin-interacting motif) repeats.,subcellular location:Localizes at sites of DNA damage at double-strand breaks (DSBs).,subunit:Interacts with TSP57 (By similarity). Component of the BRCA1-A complex, at least composed of the BRCA1, BARD1, UIMC1/RAP80, FAM175A/Abraxas, BRCC3/BRCC36, BRE/BRCC45 and MERIT40/NBA1. In the BRCA1-A complex, interacts directly with FAM175A/Abraxas. Interacts with ESR1, NR6A1 and UBE2I.,tissue specificity:Expressed in testis, ovary, thymus and heart. Expressed in germ cells of the testis., |
