Name: U-Blot® S6K1 Rabbit mAb
SKU: WR4035
Price: 268 USD
Availability: InStock

U-Blot® S6K1 Rabbit mAb

WR4035

Immunohistochemical analysis of paraffin-embedded Rat brain tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).

WR4035 Immunohistochemical analysis of paraffin-embedded Rat brain tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).

Immunohistochemical analysis of paraffin-embedded Mouse brain tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).

WR4035 Immunohistochemical analysis of paraffin-embedded Mouse brain tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).

Western blot analysis of lysates from HeLa cells, using S6K1 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.

WR4035 Western blot analysis of lysates from HeLa cells, using S6K1 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.

Price range: $268.00 through $328.00

Size

100μl

50μl

Clear

SKU: WR4035-50

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Product details

Background:ribosomal protein S6 kinase B1(RPS6KB1) Homo sapiens This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013],

Specifications

Size	50μL/100μL
Target	p70 S6 kinase α
Reactivity	Human, Mouse, Rat, Pig
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	59kD
MW(Observed)	70kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1:1000; WB 1:1000-1:5000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200, / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0095R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	RPS6KB1 STK14A P70S6K
Protein Name	Ribosomal protein S6 kinase beta-1，p70 S6 Kinase (Thr389)
Other Name	RPS6KB1; / STK14A; / Ribosomal protein S6 kinase beta-1; / S6K-beta-1; / S6K1; / 70 kDa ribosomal protein S6 kinase 1; / P70S6K1; / p70-S6K 1; / Ribosomal protein S6 kinase I; / Serine/threonine-protein kinase 14A; / p70 ribosomal S6 kinase alpha; / p70 S6 kinas
Species	Human
Gene ID	6198
Uniprot	P23443,
Species.1	Mouse
Gene ID.1	72508
Uniprot.1	Q8BSK8,
Species.2	Rat
Gene ID.2	83840
Uniprot.2	P67999
Cellular Localization	Cytoplasm
Tissue Expression	Widely expressed.
Signaling_pathway	Cellular Processes >> Transport and catabolism >> Autophagy - animal;Organismal Systems >> Immune system >> Fc gamma R-mediated phagocytosis;Organismal Systems >> Endocrine system >> Insulin signaling pathway;Organismal Systems >> Aging >> Longevity regulating pathway;Organismal Systems >> Aging >> Longevity regulating pathway - multiple species;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: overview >> PD-L1 expression and PD-1 checkpoint pathway in cancer;Human Diseases >> Cancer: specific types >> Colorectal cancer;Human Diseases >> Cancer: specific types >> Pancreatic cancer;Human Diseases >> Cancer: specific types >> Hepatocellular carcinoma;Human Diseases >> Cancer: specific types >> Gastric cancer;Human Diseases >> Cancer: specific types >> Acute myeloid leukemia;Human Diseases >> Cancer: specific types >> Breast cancer;Environmental Information Processing >> Signal transduction >> ErbB signaling pathway;Environmental Information Processing >> Signal transduction >> TGF-beta signaling pathway;Environmental Information Processing >> Signal transduction >> Apelin signaling pathway;Environmental Information Processing >> Signal transduction >> HIF-1 signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway;Environmental Information Processing >> Signal transduction >> AMPK signaling pathway;Environmental Information Processing >> Signal transduction >> mTOR signaling pathway
Research Areas
Function	Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Activation by serine/threonine phosphorylation and protein kinase C, inactivated by type 2A phosphatase.,Function:Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with PPP1R9A/neurabin-1.,tissue specificity:Widely expressed.,