Price range: $268.00 through $328.00
Background:
This gene encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | TWF1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| MW(Calculated) | 39kD |
| MW(Observed) | |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from human TWF1 AA range: 179-229 |
| Specificity | This antibody detects endogenous levels of TWF1 at Human/Mouse/Rat |
| Gene Name | TWF1 PTK9 |
| Protein Name | TWF1 |
| Other Name | |
| Species | Human |
| Gene ID | 5756 |
| Uniprot | Q12792, |
| Species.1 | Mouse |
| Gene ID.1 | 19230 |
| Uniprot.1 | Q91YR1, |
| Species.2 | Rat |
| Gene ID.2 | 315265 |
| Uniprot.2 | Q5RJR2 |
| Cellular Localization | Cytoplasm. Cytoplasm, cytoskeleton . Diffuse cytoplasmic localization with perinuclear and G-actin-rich cortical actin structures sublocalization. Also found at membrane ruffles and cell-cell contacts (By similarity). . |
| Tissue Expression | Expressed at high levels in the colon, testis, ovary, prostate and lung. Expressed at lower levels in the brain, bladder and heart. Not detected in liver. |
| Signaling_pathway | |
| Research Areas | |
| Function | Caution:Was originally (PubMed:7507208) thought to have protein tyrosine kinase activity.,Function:Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles.,online information:Molecular embrace -Issue 73 of August 2006,PTM:Phosphorylated on serine and threonine residues.,similarity:Belongs to the actin-binding proteins ADF family. Twinfilin subfamily.,similarity:Contains 2 ADF-H domains.,subcellular location:Diffuse cytoplasmic localization with perinuclear and G-actin-rich cortical actin structures sublocalization. Also found at membrane ruffles and cell-cell contacts.,subunit:Interacts with G-actin; ADP-actin form and capping protein (CP). May also be able to interact with TWF2 and phosphoinositides, PI(4,5)P2. When bound to PI(4,5)P2, it is down-regulated.,tissue specificity:Expressed at high levels in the colon, testis, ovary, prostate and lung. Expressed at lower levels in the brain, bladder and heart. Not detected in liver., |
