SKU: WP14829 Categories: ,

TPH1 Polyclonal Antibody

Price range: $268.00 through $328.00

SKU: WP14829-100
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Product details

Background:

This gene encodes a member of the aromatic amino acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in this gene have been associated with an elevated risk for a variety of diseases and disorders, including schizophrenia, somatic anxiety, anger-related traits, bipolar disorder, suicidal behavior, addictions, and others.[provided by RefSeq, Apr 2009],

Specifications

Size50μL/100μL
TargetTryptophan Hydroxylase
ReactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)51kD
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from human Tryptophan Hydroxylase. AA range:26-75
SpecificityTPH1 Polyclonal Antibody detects endogenous levels of TPH1 protein.
Gene NameTPH1
Protein NameTryptophan 5-hydroxylase 1
Other NameTPH1; / TPH; / TPRH; / TRPH; / Tryptophan 5-hydroxylase 1; / Tryptophan 5-monooxygenase 1
SpeciesHuman
Gene ID7166
UniprotP17752,
Species.1Mouse
Gene ID.121990
Uniprot.1P17532,
Species.2Rat
Gene ID.224848
Uniprot.2P09810
Cellular Localizationcytosol,neuron projection,
Tissue Expression[Isoform 2]: Seems to be less widely expressed than isoform 1.
Signaling_pathway
Research Areas>>Tryptophan metabolism; / >>Folate biosynthesis; / >>Metabolic pathways; / >>Serotonergic synapse
FunctionCatalytic activity:L-tryptophan + tetrahydrobiopterin + O(2) = 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin.,cofactor:Fe(2+) ion.,pathway:Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2.,similarity:Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.,similarity:Contains 1 ACT domain.,subunit:Multimer of identical subunits.,tissue specificity:Isoform 2 seems to be less widely expressed than isoform 1.,
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