Price range: $268.00 through $328.00
Background:
Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TRMT12 is the human homolog of a yeast gene essential for yW synthesis (Noma and Suzuki, 2006).[supplied by OMIM, Mar 2008],
| Target | TYW2 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| MW(Calculated) | 49kD |
| MW(Observed) | |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Modification | |
| Recommended Dilution Ratio | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Source | |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Purity | |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from human TYW2 AA range: 11-61 |
| Sequence | |
| Specificity | This antibody detects endogenous levels of TYW2 at Human/Mouse/Rat |
| Gene Name | TRMT12 TRM12 TYW2 |
| Protein Name | TYW2 |
| Other Name | |
| Species | Human |
| Gene ID-1 | 55039 |
| Uniprot | Q53H54, |
| Species.1 | Mouse |
| Gene ID-2 | 68260 |
| Uniprot.1 | Q8BG71, |
| Species.2 | Rat |
| Gene ID-3 | 314999 |
| Uniprot.2 | Q4V8B8 |
| Organism-4 | |
| Gene ID-4 | |
| SwissProt-4 | |
| Background | Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TRMT12 is the human homolog of a yeast gene essential for yW synthesis (Noma and Suzuki, 2006).[supplied by OMIM, Mar 2008], |
| Cellular Localization | |
| Tissue Expression | |
| Signaling_pathway | |
| Research Areas | |
| Function | Function:Probable S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA.,pathway:tRNA modification; wybutosine-tRNA(Phe) biosynthesis.,sequence Caution:Contaminating sequence. Potential poly-A sequence starting in position 371.,sequence Caution:Translation N-terminally shortened.,similarity:Belongs to the RNA methyltransferase trmD family. TYW2 subfamily., |
| RRID |
