SKU: WP15020 Categories: ,

WTAP Polyclonal Antibody

Price range: $268.00 through $328.00

SKU: WP15020-100
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Product details

Background:

The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in several transcript variants encoding three different isoforms. [provided by RefSeq, Jul 2012],

Specifications

TargetWTAP
ReactivityHuman, Mouse
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)55kD
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Modification
Recommended Dilution RatioWB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from human WTAP. AA range:321-370
Sequence
SpecificityWTAP Polyclonal Antibody detects endogenous levels of WTAP protein.
Gene NameWTAP
Protein NamePre-mRNA-splicing regulator WTAP
Other NameWTAP; / KIAA0105; / Pre-mRNA-splicing regulator WTAP; / Female-lethal; / 2; / D homolog; / hFL; / 2; / D; / WT1-associated protein; / Wilms tumor 1-associating protein
SpeciesHuman
Gene ID-19589
UniprotQ15007,
Species.1Mouse
Gene ID-260532
Uniprot.1Q9ER69
Species.2
Gene ID-3
Uniprot.2
Organism-4
Gene ID-4
SwissProt-4
BackgroundThe Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in several transcript variants encoding three different isoforms. [provided by RefSeq, Jul 2012],
Cellular LocalizationNucleus speckle . Nucleus, nucleoplasm . Cytoplasm . Mainly nuclear with some fraction located in the cytoplasm. ZC3H13 is required to anchor component of the MACOM subcomplex, such as VIRMA, in the nucleus. .
Tissue ExpressionUbiquitously expressed.
Signaling_pathway
Research Areas
FunctionFunction:Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes. May be involved in mRNA splicing regulation.,induction:In smooth muscle cells, up-regulated after serum withdrawal, when cells become mature and non proliferative.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the fl(2)d family.,subunit:Interacts with WT1.,tissue specificity:Ubiquitously expressed.,
RRID
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